Coding

Part:BBa_K4121027:Design

Designed by: Ziyu Li   Group: iGEM22_NEFU_China   (2022-10-05)


phytoene desaturase encoding gene crtI from Blakeslea trispora


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1087
    Illegal SpeI site found at 928
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 928
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1087
    Illegal SpeI site found at 928
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1087
    Illegal SpeI site found at 928
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Before using this part, give priority to whether it has the enzyme cleavage site required, if so, consider site-directed mutagenesis.


Source

National Center for Biotechnology Information (NCBI)

References